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Methods Mol Biol. 2005;291:373-84.

Measuring recombination proficiency in mouse embryonic stem cells.

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1
Markey Cancer Center, University of Kentucky, Lexington, USA.

Abstract

A method is presented to measure homologous recombination in mouse embryonic stem cells by both gene targeting and short-tract gene conversion of a double-strand break. A fluorescence-based reporter is first gene targeted to the Hprt locus in a quantifiable way. A homing endonuclease expression vector is then introduced to generate a double-strand break, the repair of which is also quantifiable.

PMID:
15502236
[Indexed for MEDLINE]
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