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Methods Mol Biol. 2005;289:121-6.

Epidermal cell analysis by RT-PCR.

Author information

1
Ottawa Health Research Institute, Ottawa, Ontario, Canada.

Abstract

Reverse transcription polymerase chain reaction is used for the semiquantitative analysis of epidermal gene expression, particularly when immunolocalization is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction method to analyze gene expression both in vivo and in vitro.

PMID:
15502177
DOI:
10.1385/1-59259-830-7:121
[Indexed for MEDLINE]

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