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Curr Biol. 2004 Oct 26;14(20):1801-11.

XRHAMM functions in ran-dependent microtubule nucleation and pole formation during anastral spindle assembly.

Author information

1
Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. aaron_groen@students.hms.harvard.edu

Abstract

BACKGROUND:

The regulated assembly of microtubules is essential for bipolar spindle formation. Depending on cell type, microtubules nucleate through two different pathways: centrosome-driven or chromatin-driven. The chromatin-driven pathway dominates in cells lacking centrosomes.

RESULTS:

Human RHAMM (receptor for hyaluronic-acid-mediated motility) was originally implicated in hyaluronic-acid-induced motility but has since been shown to associate with centrosomes and play a role in astral spindle pole integrity in mitotic systems. We have identified the Xenopus ortholog of human RHAMM as a microtubule-associated protein that plays a role in focusing spindle poles and is essential for efficient microtubule nucleation during spindle assembly without centrosomes. XRHAMM associates both with gamma-TuRC, a complex required for microtubule nucleation and with TPX2, a protein required for microtubule nucleation and spindle pole organization.

CONCLUSIONS:

XRHAMM facilitates Ran-dependent, chromatin-driven nucleation in a process that may require coordinate activation of TPX2 and gamma-TuRC.

PMID:
15498487
DOI:
10.1016/j.cub.2004.10.002
[Indexed for MEDLINE]
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