Format

Send to

Choose Destination
Genomics. 2004 Sep;84(3):550-4.

Chromosome-specific microsatellite multiplex sets for linkage studies in the domestic dog.

Author information

1
Department of Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.

Abstract

To expedite linkage studies and positional cloning efforts in the dog, Minimal Screening Set 2 (MSS-2) of 327 canine microsatellite markers has been multiplexed into chromosome-specific panels. MSS-2 provides 9 Mb coverage of the canine genome with no gaps larger than 17.1 Mb and is the most recent and comprehensive set of microsatellites available for whole-genome scans. Markers were labeled with fluorescent dyes based on locations and expected product sizes to facilitate the multiplexing of a maximum number of markers for each chromosome. All markers are amplified using a single thermal cycling program and PCR mix and are optimized for resolution on an ABI 3100 genetic analyzer. Sixty-nine chromosome-specific panels were created by coamplification of a maximum number of markers and subsequent coloading of the remaining markers.

PMID:
15498461
DOI:
10.1016/j.ygeno.2004.06.006
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center