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Mol Cell. 2004 Oct 22;16(2):235-44.

Modulation of transcription affects mRNP quality.

Author information

1
Department of Molecular Biology, Aarhus University, C.F. Møllers Alle, Building 130, 8000 Aarhus C, Denmark. thj@mb.au.dk

Abstract

Cotranscriptional loading of proteins onto nascent transcripts contributes to the formation of messenger ribonucleoprotein particles (mRNPs) competent for nuclear export. The transcription machinery is believed to play a pivotal role in mRNP assembly, which is at least partially linked to the function of the THO/TREX complex and the mRNA termination/polyadenylation apparatus. Here we demonstrate a prominent role for the rate of transcription in the production of export-competent mRNPs. We show that a transcription-defective allele of the Rad3p helicase, a component of the TFIIH transcription initiation factor, suppresses several phenotypes associated with defective mRNA processing and export. Strikingly, the effects of compromised Rad3p activity can be phenocopied by a transcription elongation drug as well as by other mutations affecting transcription. Our results suggest that efficient mRNP assembly is under a kinetic control that is influenced by the rate of transcription.

PMID:
15494310
DOI:
10.1016/j.molcel.2004.09.019
[Indexed for MEDLINE]
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