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Biosens Bioelectron. 2004 Oct 15;20(3):509-17.

Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics.

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1
Department of Chemistry and Biochemistry, School of Medicine, The California NanoSystems Institute, University of California, Los Angeles, CA 90095-1569, USA.

Abstract

We report on a silicon wafer-based device that can be used for recording macroscopic ion channel protein activities across a diverse group of cell-types. Gigaohm seals were achieved for CHO-K1 and RIN m5F cells, and both cell-attached and whole-cell mode configurations were also demonstrated. Two distinct intrinsic potassium ion channels were recorded in whole-cell mode for HIT-T15 and RAW 264.7 cells. Polydimethylsiloxane (PDMS) microfluidics were also coupled with the micromachined silicon chips in order to demonstrate that a single cell could be selectively directed to a micropore, and membrane protein currents could subsequently be recorded. These silicon chip-based devices have significant advantages over traditional micropipette approaches, and may serve as combinatorial tools for investigating membrane biophysics, pharmaceutical screening, and other bio-sensing tasks.

PMID:
15494233
DOI:
10.1016/j.bios.2004.02.020
[Indexed for MEDLINE]
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