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Proc Natl Acad Sci U S A. 2004 Oct 26;101(43):15275-8. Epub 2004 Oct 18.

Triggered amplification by hybridization chain reaction.

Author information

1
Department of Chemistry, California Institute of Technology, Pasadena, CA 91125, USA.

Abstract

We introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridization events that yields nicked double helices analogous to alternating copolymers. The average molecular weight of the HCR products varies inversely with initiator concentration. Amplification of more diverse recognition events can be achieved by coupling HCR to aptamer triggers. This functionality allows DNA to act as an amplifying transducer for biosensing applications.

PMID:
15492210
PMCID:
PMC524468
DOI:
10.1073/pnas.0407024101
[Indexed for MEDLINE]
Free PMC Article

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