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J Chromatogr A. 2004 Sep 10;1048(2):195-8.

Simple reversed-phase liquid chromatography method for determination of tocopherols in edible plant oils.

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  • 1Faculty of Commodity Science, Poznań University of Economics, al Niepodległości 10, 60-967 Poznań, Poland.


A simple and rapid reversed-phase high-performance liquid chromatography method for determination of alpha-, (beta + gamma), and delta-tocopherols in edible plant oils has been developed. Oils are diluted in 2-propanol and injected directly onto Symmetry C18 column. Methanol and acetonitrile (1:1) are used as a mobile phase. Tocopherols are detected using fluorescence detector set at excitation and emission wavelength 295 nm and 325 nm, respectively. The method is precise (R.S.D. not higher than 2.24%) and sensitive-detection limits (DL) are 8 ng/ml for gamma- and delta-tocopherols and 28 ng/ml for alpha-tocopherol; quantification limits (QL) were calculated as three times higher than DL.

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