Format

Send to

Choose Destination
Gene. 1992 Feb 1;111(1):143-4.

A modified kanamycin-resistance cassette to facilitate two-codon insertion mutagenesis.

Author information

1
Department of Microbiology and Immunology, SUNY-Health Science Center, Brooklyn 11203-2098.

Abstract

A kanamycin-resistance (KmR) cassette was modified to allow its introduction as a blunt-ended DNA fragment into target plasmids. Subsequent excision of the KmR cassette with ApaI and religation of the plasmid leaves behind six base pairs (5'-GGGCCC) that result in the insertion of two amino acids in the target protein.

PMID:
1547951
DOI:
10.1016/0378-1119(92)90617-x
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center