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Biochim Biophys Acta. 1992 Mar 12;1119(3):256-60.

Crystalline enzyme kinetics: activity of the Streptomyces R61 D-alanyl-D-alanine peptidase.

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1
Sir William Dunn School of Pathology, University of Oxford, U.K.

Abstract

The specificity constant, kcat/Km, for the hydrolysis of hippuryl-mercaptoacetate by crystals of the Streptomyces R61 D-D peptidase was measured by reaction of the thiol produced with 4,4'-dithiodipyridine. The values of kcat/Km for the crystal and in solution were the same (within experimental error). A novel method for treating the lag in the progress curves was developed.

PMID:
1547270
[Indexed for MEDLINE]

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