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J Antimicrob Chemother. 2004 Nov;54(5):932-5. Epub 2004 Oct 7.

Identification of a chromosome-borne class C beta-lactamase from Erwinia rhapontici.

Author information

1
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. thierry.naas@bct.ap-hop-paris.fr

Abstract

OBJECTIVES:

To characterize the beta-lactamase gene content of Erwinia rhapontici.

METHODS:

The beta-lactamase gene was cloned, sequenced and expressed in Escherichia coli.

RESULTS:

The cloned gene conferred a resistance pattern of an Ambler class C beta-lactamase in E. coli. The AmpC-type enzyme had a pI value of 8.6 and shared 62% amino acid sequence identity with that of Escherichia fergusonii. The ampC gene was associated with a regulatory ampR gene and beta-lactamase production was inducible.

CONCLUSIONS:

This work provides further evidence of the molecular heterogeneity of beta-lactamases in Erwinia spp. and that plant-pathogenic enterobacterial species may constitute a reservoir of antibiotic resistance genes.

PMID:
15472000
DOI:
10.1093/jac/dkh446
[Indexed for MEDLINE]

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