Format

Send to

Choose Destination
See comment in PubMed Commons below
J Cyst Fibros. 2004 Aug;3 Suppl 2:159-63.

Ex vivo CF diagnosis by intestinal current measurements (ICM) in small aperture, circulating Ussing chambers.

Author information

1
Departments of Paediatric Gastroenterology and Biochemistry, Erasmus University Medical Centre, P.O. Box 1738, Rotterdam, 3000DR The Netherlands. h.dejonge@erasmusmc.nl

Abstract

Intestinal current measurements (ICM) on rectal suction biopsies are a tool for the ex vivo diagnosis of classical and atypical cystic fibrosis (CF). We present the basic ICM protocol, typical tracings and their interpretation. The ICM technique allows the registration of CF-induced changes in electrogenic transepithelial ion transport (Cl-, HCO3-, K+) in a Cl- secretory epithelium, and on the basis of pharmacological criteria, is able to discriminate between CFTR-mediated Cl- secretion and secretion through alternative anion channels. ICM is particularly useful for the classification of individuals with CF-like clinical features with equivocal sweat test values and/or no or one identifiable CFTR mutation.

PMID:
15463951
DOI:
10.1016/j.jcf.2004.05.034
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center