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J Cyst Fibros. 2004 Aug;3 Suppl 2:73-7.

Biochemical methods to assess CFTR expression and membrane localization.

Author information

1
Centre of Human Genetics, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal. cfarinha@igc.gulbenkian.pt

Abstract

Detection of cystic fibrosis transmembrane conductance regulator (CFTR) protein is usually a difficult task to accomplish due to the low levels of expression and high turnover that this membrane protein is submitted to in the cell. Common biochemical methods can be used for the detection of CFTR but several critical points must be taken into account. The scope of this article is to outline biochemical methods commonly used to assess CFTR expression, processing and membrane localization.

PMID:
15463932
DOI:
10.1016/j.jcf.2004.05.017
[Indexed for MEDLINE]
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