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J Cyst Fibros. 2004 Aug;3 Suppl 2:17-23.

Quantitative methods for the analysis of CFTR transcripts/splicing variants.

Author information

1
Department of Chemistry and Biochemistry, University of Lisboa, Lisboa, Portugal. mdamaral@fc.ul.pt

Abstract

In cystic fibrosis (CF), transcript analysis and quantification are important for diagnosis, prognosis and also as surrogate markers for some therapies including gene therapy. Classical RNA-based methods require significant expression levels in target samples for appropriate analysis, thus PCR-based methods are evolving towards reliable quantification. Various protocols for the quantitative analysis of CFTR transcripts (including those resulting from splicing variants) are described and discussed here.

PMID:
15463919
DOI:
10.1016/j.jcf.2004.05.047
[Indexed for MEDLINE]
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