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Clin Chem. 2004 Dec;50(12):2353-60. Epub 2004 Sep 30.

Photonic crystal glucose-sensing material for noninvasive monitoring of glucose in tear fluid.

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  • 1Department of Chemistry, Chevron Science Center, Department of Pediatrics, University of Pittsburgh Medical School, University of Pittsburgh, Pittsburgh, PA 15260, USA.

Abstract

BACKGROUND:

We recently developed a photonic crystal glucose-sensing material, which consists of a crystalline colloidal array embedded within a polymer network of a polyacrylamide-poly(ethylene glycol) hydrogel with pendent phenylboronic acid groups. The aim of the present work was to improve this approach for application to noninvasive or minimally invasive monitoring of glucose.

METHODS:

We used new boronic acid derivatives such as 4-amino-3-fluorophenylboronic acid and 4-carboxy-3-fluorophenylboronic acid as the molecular recognition elements to achieve sensing at physiologic pH values.

RESULTS:

The improved photonic glucose-sensing material sensed glucose in the range of the 100 mumol/L concentrations found in tear fluid. The detection limits were approximately 1 mumol/L in synthetic tear fluid. The visually evident diffraction color shifted across the entire visible spectral region from red to blue over the physiologically relevant tear-fluid glucose concentrations. This sensing material is selective for glucose over galactose, mannose, and fructose.

CONCLUSIONS:

These new glucose sensors have properties appropriate for use in such glucose-sensing applications as ocular inserts or diagnostic contact lenses for patients with diabetes mellitus.

PMID:
15459093
DOI:
10.1373/clinchem.2004.039701
[PubMed - indexed for MEDLINE]
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