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Bull Exp Biol Med. 2004 Jun;137(6):635-8.

Spatial characteristics of cisterna magna in rats and novel technique for puncture with a stereotactic manipulator.

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V. P. Serbskii State Research Center for Social and Forensic Psychiatry, Moscow, Russia.


The actual size and sites for puncture of the cisterna magna in adult Wistar rats were estimated on acrylic models. We proposed a new method for obtaining cerebrospinal fluid from narcotized rats. This method consists in controlled introduction of a needle into the cisterna magna through the skin and/or dura mater above this structure using a stereotactic micromanipulator. Experiments on rats with focal and global cerebral ischemia showed that this technique ensures single and repeated sampling of at least 50 microl liquor not containing blood admixtures.

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