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FEMS Microbiol Lett. 2004 Oct 1;239(1):71-7.

Analysis of the germination of spores of Bacillus subtilis with temperature sensitive spo mutations in the spoVA operon.

Author information

1
Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032, USA.

Abstract

A Bacillus subtilis strain with a base substitution in the ribosome-binding site of spoVAC was temperature sensitive (ts) in sporulation and spores prepared at the permissive temperature were ts in L-alanine-triggered germination, but not in germination with Ca2+-dipicolinic acid (DPA) or dodecylamine. Spores of a ts spo mutant with a missense mutation in the spoVAC coding region were not ts for germination with l-alanine, dodecylamine or Ca2+-DPA. These findings are discussed in light of the proposal that SpoVA proteins are involved not only in DPA uptake during sporulation, but also in DPA release during nutrient-mediated spore germination.

PMID:
15451103
DOI:
10.1016/j.femsle.2004.08.022
[Indexed for MEDLINE]
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