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Protein Sci. 2004 Oct;13(10):2825-8.

Average protein density is a molecular-weight-dependent function.

Author information

1
Institute of Physics, University of São Paulo, Rua do Matao Travessa R, 187 CEP 05508-900, São Paulo-SP, Brazil.

Abstract

The mass density of proteins is a relevant basic biophysical quantity. It is also a useful input parameter, for example, for three-dimensional structure determination by protein crystallography and studies of protein oligomers in solution by analytic ultracentrifugation. We have performed a critical analysis of published, theoretical, and experimental investigations about this issue and concluded that the average density of proteins is not a constant as often assumed. For proteins with a molecular weight below 20 kDa, the average density exhibits a positive deviation that increases for decreasing molecular weight. A simple molecular-weight-depending function is proposed that provides a more accurate estimate of the average protein density.

PMID:
15388866
PMCID:
PMC2286542
DOI:
10.1110/ps.04688204
[Indexed for MEDLINE]
Free PMC Article

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