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Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography-ultraviolet detection analysis.

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1
Instituto de Investigación en Recursos Cinegéticos, CSIC-UCLM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. rafael.mateo@uclm.es

Abstract

Methods developed for porphyrin analysis have low recoveries and/or poor precision for the less polar protoporphyrin IX. We describe a simple method of analysis of porphyrins and biliverdin in bile and excreta of birds based on extraction with HCl 3N: acetonitrile and HPLC/UV analyses. Recoveries were good for protoporphyrin IX and other porphyrins (>79%). Applications of this method showed that porphyrins and biliverdin in birds excreta are mainly of biliary-fecal origin rather than urinary origin. Biliverdin and protoporphyrin IX increased proportionately more than the rest of the porphyrins and coproporphyrin III increased more than coproporphyrin I in the bile of Pb-poisoned mallards.

PMID:
15380729
DOI:
10.1016/j.jchromb.2004.08.019
[Indexed for MEDLINE]
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