Format

Send to

Choose Destination
J Infect Dis. 2004 Oct 15;190(8):1396-403. Epub 2004 Sep 8.

Frequent hepatitis C virus superinfection in injection drug users.

Author information

1
Blood Systems Research Institute, University of California, San Francisco, Califronia, USA.

Abstract

The frequency of hepatitis C virus (HCV) superinfection with a divergent viral strain was determined in a cohort of recently infected young injection drug users (IDUs) with an HCV incidence rate of 25%. HCV was amplified, by use of polymerase chain reaction (PCR), from plasma samples collected from 25 HCV-infected individuals over an average period of 12 months, and their viral sequences were compared. Phylogenetic analysis identified 5 IDUs with superinfection (20%) occurring after seroconversion: 2 IDUs were superinfected with different HCV genotypes, and 3 were superinfected with divergent strains of the same genotype. The superinfecting strains were not detected as minority variants (<0.5%) in the initial plasma HCV quasi species. Extensive measures were taken to exclude PCR contamination and mix-up of samples, and superinfection results were concordant at 2 HCV genetic loci. HCV superinfection in IDUs, both intra- and intergenotype, is therefore a frequent event, with an incidence rate similar to that of de novo infections. These results suggest that no cross-protecting immunity develops during the first year of chronic infection with HCV.

PMID:
15378431
DOI:
10.1086/424491
[Indexed for MEDLINE]

Publication types, MeSH terms, Secondary source ID, Grant support

Publication types

MeSH terms

Secondary source ID

Grant support

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center