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Bioinformatics. 2005 Jan 15;21(2):257-9. Epub 2004 Sep 17.

RALEE--RNA ALignment editor in Emacs.

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  • 1The Wellcome Trust Sanger Institute Wellcome Trust Genome Campus, Hinxton, CAMBS, CB10 1SA, UK.


Production of high quality multiple sequence alignments of structured RNAs relies on an iterative combination of manual editing and structure prediction. An essential feature of an RNA alignment editor is the facility to mark-up the alignment based on how it matches a given secondary structure prediction, but few available alignment editors offer such a feature. The RALEE (RNA ALignment Editor in Emacs) tool provides a simple environment for RNA multiple sequence alignment editing, including structure-specific colour schemes, utilizing helper applications for structure prediction and many more conventional editing functions. This is accomplished by extending the commonly used text editor, Emacs, which is available for Linux, most UNIX systems, Windows and Mac OS.


The ELISP source code for RALEE is freely available from along with documentation and examples.


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