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J Microbiol Methods. 2004 Nov;59(2):289-92.

Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells.

Author information

1
Department of Laboratory Medicine and Pathology, Box 609 Mayo Building, 420 Delaware Street, S.E., University of Minnesota, Minneapolis 55455, USA.

Abstract

The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures.

PMID:
15369865
DOI:
10.1016/j.mimet.2004.07.001
[Indexed for MEDLINE]

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