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J Proteome Res. 2004 Jul-Aug;3(4):736-42.

Comparison of multiplexed techniques for detection of bacterial and viral proteins.

Author information

1
Lawrence Livermore National Laboratory, Livermore, California 94550, USA.

Abstract

Immobilized antibody microarrays were compared to the Luminex flow cytometry system that utilizes suspensions of polystyrene microbeads covalently coupled with capture antibodies. The two immunoassays were performed for comparison of reproducibility, limits of detection and dynamic range. The Luminex system showed lower limits of detection and increased dynamic range among samples whereas the protein microarrays could be more amenable to miniaturization. Both technologies were capable of sensitive multiplexed detection.

PMID:
15359726
DOI:
10.1021/pr034130t
[Indexed for MEDLINE]

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