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Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13501-6. Epub 2004 Sep 7.

The inositol polyphosphate 5-phosphatase Ocrl associates with endosomes that are partially coated with clathrin.

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1
Division of Hematology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Abstract

The subcellular localization of Ocrl, the inositol polyphosphate 5-phosphatase that is mutated in Lowe syndrome, was investigated by fluorescence microscopy. Ocrl was localized to endosomes and Golgi membranes along with clathrin, giantin, the mannose 6-phosphate receptor, transferrin, and the early endosomal antigen 1 endosomal marker in fixed cells. The endosomal localization of Ocrl was confirmed by live-cell time-lapse microscopy in which we monitored the dynamics of Ocrl on endosomes. GST binding assays show that Ocrl interacts with the clathrin terminal domain and the clathrin adaptor protein AP-2. Our findings suggest a role for Ocrl in endosomal receptor trafficking and sorting.

PMID:
15353600
PMCID:
PMC518786
DOI:
10.1073/pnas.0405664101
[Indexed for MEDLINE]
Free PMC Article
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