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Biochem Biophys Res Commun. 2004 Oct 8;323(1):156-62.

Allogenic antibody-mediated identification of head and neck cancer antigens.

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  • 1Department of Head and Neck Surgery, Ludwig-Maximilians-Universit√§t M√ľnchen, Marchioninistr.15, D-81377 Munich, Germany.

Abstract

Recently, we described a new target-identification technology, autoantibody-mediated identification of antigens (AMIDA). AMIDA takes advantage of autologous serum autoantibodies to identify disease-associated antigens. Here, we evaluated the allogenic variant of AMIDA (allo-AMIDA), using permanent cancer cell lines as an antigen-pool rather than primary biopsy samples. Twelve different proteins were retrieved exclusively with antibodies from cancer patients, but not from healthy donors. The expression of three of these antigens, e-FABP, hnRNP H, and Grb2, was evaluated in more detail. All three proteins were strongly overexpressed in primary carcinomas and metastases thereof, as compared to healthy epithelium. Additionally, serum reactivity against e-FABP was detected in 20% of cancer patients but only 2% of healthy volunteers. In summary, we demonstrate that permanent cancer cell lines represent a reliable source for tumour-associated antigens. Moreover, we show that allo-AMIDA is suitable for the identification of tumour-specific antigens overcoming the limitations of autologous screening techniques.

PMID:
15351715
DOI:
10.1016/j.bbrc.2004.08.071
[PubMed - indexed for MEDLINE]
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