Alternative modalities of adenovirus-mediated gene expression in hippocampal neurons cultured on microisland substrate

Neurosci Lett. 2004 Sep 23;368(2):221-5. doi: 10.1016/j.neulet.2004.07.022.

Abstract

Previously, we have used CsCl gradient-purified recombinant adenovirus (AdV) to successfully transfer genes into hippocampal neurons cultured on microisland substrate. Here, we report that purification of AdV particles is not required and efficient gene expression can be achieved using either crude AdV lysates or HEK 293 cells infected with AdV. The advantages of the simplified procedure are greatly reduced preparation time and reduced requirements for equipment and expertise.

Publication types

  • Comparative Study

MeSH terms

  • Adenoviridae / genetics*
  • Adenoviridae / metabolism
  • Animals
  • Animals, Newborn
  • Baclofen / pharmacology
  • Cells, Cultured
  • Drug Interactions
  • Embryo, Mammalian
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / genetics
  • GABA Agonists / pharmacology
  • GTP-Binding Protein alpha Subunits, Gq-G11 / pharmacology
  • Gene Expression / physiology*
  • Gene Transfer Techniques
  • Genetic Vectors
  • Hippocampus / cytology*
  • Hippocampus / virology
  • Humans
  • Membrane Potentials / genetics
  • Neurons / metabolism*
  • Neurons / virology
  • Patch-Clamp Techniques
  • Pertussis Toxin / pharmacology
  • Rats
  • Rats, Sprague-Dawley

Substances

  • GABA Agonists
  • Pertussis Toxin
  • GTP-Binding Protein alpha Subunits, Gq-G11
  • Baclofen