Abstract
Previously, we have used CsCl gradient-purified recombinant adenovirus (AdV) to successfully transfer genes into hippocampal neurons cultured on microisland substrate. Here, we report that purification of AdV particles is not required and efficient gene expression can be achieved using either crude AdV lysates or HEK 293 cells infected with AdV. The advantages of the simplified procedure are greatly reduced preparation time and reduced requirements for equipment and expertise.
MeSH terms
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Adenoviridae / genetics*
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Adenoviridae / metabolism
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Animals
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Animals, Newborn
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Baclofen / pharmacology
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Cells, Cultured
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Drug Interactions
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Embryo, Mammalian
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Excitatory Postsynaptic Potentials / drug effects
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Excitatory Postsynaptic Potentials / genetics
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GABA Agonists / pharmacology
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GTP-Binding Protein alpha Subunits, Gq-G11 / pharmacology
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Gene Expression / physiology*
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Gene Transfer Techniques
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Genetic Vectors
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Hippocampus / cytology*
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Hippocampus / virology
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Humans
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Membrane Potentials / genetics
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Neurons / metabolism*
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Neurons / virology
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Patch-Clamp Techniques
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Pertussis Toxin / pharmacology
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Rats
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Rats, Sprague-Dawley
Substances
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GABA Agonists
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Pertussis Toxin
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GTP-Binding Protein alpha Subunits, Gq-G11
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Baclofen