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J Biochem Biophys Methods. 2004 Sep 30;60(3):265-80.

Peptide separation by Hydrophilic-Interaction Chromatography: a review.

Author information

1
Tosoh Analysis and Research Center, 2743-1 Hayakawa, Ayase-shi, Kanagawa 252-1123, Japan.

Abstract

Recent developments in the separation of peptides by high-performance liquid chromatography (HPLC) using polar sorbents with less polar eluents are summarized in this review. This separation mode is now commonly referred to as Hydrophilic-Interaction Chromatography (HILIC). The retention mechanism and chromatographic behavior of polar solutes under HILIC conditions are studied on TSKgel Amide-80 columns, which consist of carbamoyl groups bonded to a silica gel matrix, using a mixture of acetonitrile (MeCN)-water containing 0.1% trifluoroacetic acid (TFA). Some applications are given in peptide field using Hydrophilic-Interaction Chromatography.

PMID:
15345295
DOI:
10.1016/j.jbbm.2004.01.006
[Indexed for MEDLINE]

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