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Yonsei Med J. 2004 Aug 31;45(4):755-8.

Peptide mimotopes of Neisseria meningitidis group B capsular polysaccharide.

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1
Department of Microbiology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 134 Shin chon-dong, Seodaemoon-gu, Seoul 120-752, Korea.

Abstract

The antigenic similarity between Neisseria meningitidis group B (NMGB) capsular polysaccharide (PS) and human polysialic acid (PSA) has hampered the development of a NMGB PS-based vaccine. But the possibility of a safe vaccine based on NMGB PS has been demonstrated by the existence of the NMGB PS-associated nonautoreactive epitope, which is distinct from those present on human PSA. To obtain peptide mimotopes of NMGB PS, we used HmenB3, a protective and nonautoreactive monoclonal antibody, to screen a phage library with 12 amino acids. We obtained 23 phage clones that bound to HmenB3 but not in the presence of E. coli K1 PS [which is alpha(2-8)-linked PSA like NMGB PS]. The clones contained 3 mimotopes and differed from previously described NMGB PS mimotopes. Immunization with a synthetic peptide of one mimotope elicited anti-NMGB antibodies in BALB/c mice. These mimotopes may be useful in the development of group B meningococcal vaccines.

PMID:
15344223
DOI:
10.3349/ymj.2004.45.4.755
[Indexed for MEDLINE]
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