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Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1611-3. Epub 2004 Aug 26.

Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription factor DksA from Escherichia coli.

Author information

1
Genomic Sciences Center, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi Yokohama 230-0045, Japan.

Abstract

The Escherichia coli gene encoding a regulator of stringent response and virulence, DksA, which contains a canonical Zn finger motif, was cloned and expressed, and the purified protein was crystallized by the hanging-drop vapor-diffusion technique in two different space groups, P2(1)2(1)2(1) (a = 91.32, b = 96.59, c = 117.48 A) and C222 (a = 80.6, b = 115.1, c = 149.57 A). The crystals belonging to space group P2(1)2(1)2(1), improved by macroseeding, diffract beyond 2.0 A at a synchrotron. Three complete atomic resolution multiple anomalous dispersion diffraction data sets were collected from the same crystal of the P2(1)2(1)2(1) crystal form at the absorption edge for Zn atoms.

PMID:
15333933
DOI:
10.1107/S0907444904015033
[Indexed for MEDLINE]

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