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J Am Chem Soc. 2004 Sep 1;126(34):10512-3.

Micropatterns of spores displaying heterologous proteins.

Author information

1
Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Korea.

Erratum in

  • J Am Chem Soc. 2005 Jun 15;127(23):8580. Choi, Soo-Keun [added]; Jung, Heung-Chae [added]; Pan, Jae-Gu [added].

Abstract

Micropatterns of Bacillus thuringiensis spores were generated by a combination of surface chemistry, microcontact printing, and spore surface display technique. The outermost layers of B. thuringiensis spores were engineered to present enhanced green fluorescent protein (EGFP), and the biospecific interaction between biotin and streptavidin was utilized to spatially direct the EGFP-presenting spores onto the micropatterned surfaces. The viability of the micropatterned spores was confirmed by the pattern generation of vegetative cells after the germination.

PMID:
15327288
DOI:
10.1021/ja047894y
[Indexed for MEDLINE]

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