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Biosci Biotechnol Biochem. 2004 Aug;68(8):1801-4.

Expression and purification of the Bacillus subtilis thioredoxin superfamily protein YkvV.

Author information

1
Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

Abstract

Bacillus subtilis YkvV protein, an extracellular thioredoxin superfamily protein, was successfully expressed both in Brevibacillus choshinensis culture medium using an efficient promoter and the secretion signal of its surface layer protein, and in Escherichia coli cytoplasm with the amino-terminal His-tag (His-YkvV). His-YkvV was purified to homogeneity by Ni-NTA column. Both secreted YkvV and purified His-YkvV exhibited thiol-disulfide oxidoreductase activity.

PMID:
15322369
DOI:
10.1271/bbb.68.1801
[Indexed for MEDLINE]
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