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Methods Mol Biol. 2004;278:353-78.

Automated NMR structure calculation with CYANA.

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  • 1RIKEN Genomic Sciences Center, Yokohama, Japan.

Abstract

This chapter gives an introduction to automated nuclear magnetic resonance (NMR) structure calculation with the program CYANA. Given a sufficiently complete list of assigned chemical shifts and one or several lists of cross-peak positions and columns from two-, three-, or four-dimensional nuclear Overhauser effect spectroscopy (NOESY) spectra, the assignment of the NOESY cross-peaks and the three-dimensional structure of the protein in solution can be calculated automatically with CYANA.

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