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Methods Mol Biol. 2004;278:107-21.

Projection angle restraints for studying structure and dynamics of biomolecules.

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1
Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.

Abstract

This chapter presents a methodology that allows for the structural and dynamic characterization of biomolecules by means of projection restraints obtained from residual magnetic dipolar couplings. Dipolar couplings reflect the projection of individual internuclear vectors onto the alignment tensor. This technique allows determination of the dynamics of the protein backbone on time-scales, namely, between the rotational tumbling correlation time and approx 50 micros. This range of time-scales has been previously inaccessible by other nuclear magnetic resonance (NMR) techniques. In addition, information about the anisotropy of the motion is obtained.

PMID:
15317994
DOI:
10.1385/1-59259-809-9:107
[Indexed for MEDLINE]
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