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Plant J. 2004 Sep;39(5):761-75.

A compendium of methods useful for characterizing Arabidopsis pollen mutants and gametophytically-expressed genes.

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Plant Gene Expression Center and Plant and Microbial Biology, USDA/ARS and UC-Berkeley, 800 Buchanan St., Albany, CA 94710, USA.


This article provides detailed protocols for collecting pollen and outlines genetic crosses and phenotypic assays that are useful for characterizing mutants that affect pollen development.

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