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J Virol. 2004 Sep;78(17):9560-3.

Human immunodeficiency virus type 1 matrix inhibits and confers cooperativity on gag precursor-membrane interactions.

Author information

1
Aaron Diamond AIDS Research Center and the Rockefeller University, New York, New York 10016, USA.

Abstract

Human immunodeficiency virus type 1 (HIV-1) Gag multimerization and membrane binding are required for particle formation. However, it is unclear what constitutes a minimal plasma membrane-specific targeting signal and what role the matrix (MA) globular head and other Gag domains play in membrane targeting. Here, we use membrane flotation and microscopic analysis of Gag deletion mutants to demonstrate that the HIV-1 MA globular head inhibits a plasma membrane-specific targeting signal contained within the six amino-terminal MA residues. MA-mediated inhibition is relieved by concentration-dependent Gag multimerization and imparts a high degree of cooperativity on Gag-membrane association. This cooperativity may confer temporal and spatial regulation on HIV-1 assembly.

PMID:
15308748
PMCID:
PMC506924
DOI:
10.1128/JVI.78.17.9560-9563.2004
[Indexed for MEDLINE]
Free PMC Article

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