Format

Send to

Choose Destination
Acta Crystallogr D Biol Crystallogr. 1997 Mar 1;53(Pt 2):203-6.

Potential use of real-space refinement in protein structure determination.

Author information

1
Department of Chemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee 32306-3015, USA. chapman@sb.fsu.edu

Abstract

Through testing refinement protocols using free R-factor estimates of model quality, it is shown that real-space refinement can be a useful addition to conventional reciprocal-space refinement, even for protein structures with poor electron-density maps derived from multiple isomorphous replacement. By alternating real- and reciprocal-space refinements, starting with an experimental map, then calculating 2F(o) - F

PMID:
15299957
DOI:
10.1107/S0907444996012280

Supplemental Content

Loading ...
Support Center