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EMBO J. 2004 Aug 18;23(16):3375-85. Epub 2004 Aug 5.

A dedicated translation factor controls the synthesis of the global regulator Fis.

Author information

1
School of Biological Sciences, University of Southampton, Southampton, UK.

Abstract

BipA is a highly conserved protein with global regulatory properties in Escherichia coli. We show here that it functions as a translation factor that is required specifically for the expression of the transcriptional modulator Fis. BipA binds to ribosomes at a site that coincides with that of elongation factor G and has a GTPase activity that is sensitive to high GDP:GTP ratios and stimulated by 70S ribosomes programmed with mRNA and aminoacylated tRNAs. The growth rate-dependent induction of BipA allows the efficient expression of Fis, thereby modulating a range of downstream processes, including DNA metabolism and type III secretion. We propose a model in which BipA destabilizes unusually strong interactions between the 5' untranslated region of fis mRNA and the ribosome. Since BipA spans phylogenetic domains, transcript-selective translational control for the 'fast-track' expression of specific mRNAs may have wider significance.

PMID:
15297874
PMCID:
PMC514516
DOI:
10.1038/sj.emboj.7600343
[Indexed for MEDLINE]
Free PMC Article

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