Format

Send to

Choose Destination
See comment in PubMed Commons below
Mol Cell Probes. 2004 Oct;18(5):307-12.

Rapid detection and differentiation of Bartonella spp. by a single-run real-time PCR.

Author information

1
Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy. ciervo@iss.it

Abstract

A Real-time PCR for the identification and differentiation of Bartonella spp. based on the detection of mutations in an internal region of the gltA gene by thermal analysis was developed. The assay included a simultaneous detection of the amplicons by direct hybridization of LCRed and fluorescein-labelled probes coupled with melting curve analysis by the use of fluorescence resonance energy transfer technology. The protocol allowed establishing genospecie identity in less than 1 h without a need for restriction enzyme digestion or gel electrophoresis. The method is simple, reproducible, rapid, specific and potentially transferable to clinical samples.

PMID:
15294318
DOI:
10.1016/j.mcp.2004.04.004
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center