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J Assist Reprod Genet. 2004 May;21(5):169-73.

The effect of a two-hour, room temperature incubation of human spermatozoa in TEST-yolk buffer on the rate of fertilization in vitro.

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1
California Polytechnic State University, San Luis Obispo, California, USA.

Abstract

PURPOSE:

To reassess the use of TEST-yolk buffer (TYB) in an in vitro fertilization (IVF) program by comparing fertilization rates achieved in a glucose-free cleavage medium by the standard IVF preparation of sperm versus a 2-h, room temperature incubation of sperm in TYB.

METHODS:

Oocytes collected for IVF were randomly split into two groups and inseminated with either TYB-treated sperm or IVF-prepared sperm.

SETTING:

Stanford Reproductive Endocrinology and Infertility Center.

PATIENTS:

Fifty couples undergoing IVF with at least 10 mature oocytes.

MAIN OUTCOME MEASURES:

Fertilization rates in vitro.

RESULTS:

Fertilization rates were significantly higher (p = 0.015) with TYB treatment. The average 2PN fertilization rate was 49.6% (188/379) for the IVF group and 57.4% (221/385) in the IVF with TYB group.

CONCLUSIONS:

A 2-h, room temperature incubation of sperm in TYB produces significantly higher 2PN fertilization rates as compared to standard IVF preparation of sperm in a current generation cleavage medium.

[Indexed for MEDLINE]
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