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J Immunol Methods. 2004 Jul;290(1-2):69-80.

Yeast display of antibody fragments: a discovery and characterization platform.

Author information

1
Pacific Northwest National Laboratory, MSIN:K4-12, 902 Battelle Boulevard, P.O. Box 999, Richland, WA 99352, USA. feldhaus.michael@pnl.gov

Abstract

Yeast display of antibody fragments has proven to be an efficient and productive means for directed evolution of single-chain Fv (scFv) antibodies for increased affinity and thermal stability and, more recently, for the display and screening of a non-immune scFv and immune Fab libraries. A major strength of yeast display as a novel antibody discovery platform is the ability to characterize the binding properties, i.e., the affinity and epitope binding characteristics, of a clone without the need for subcloning, expression and purification of the scFv. This review focuses on novel attributes of yeast display for antibody engineering endeavors.

PMID:
15261572
DOI:
10.1016/j.jim.2004.04.009
[Indexed for MEDLINE]

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