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Cancer Cell. 2004 Jul;6(1):33-43.

Small molecule blockade of transcriptional coactivation of the hypoxia-inducible factor pathway.

Author information

1
Dana-Farber Cancer Institute and Harvard Medical School, Massachusetts 02115, USA. andrew_kung@dfci.harvard.edu

Abstract

Homeostasis under hypoxic conditions is maintained through a coordinated transcriptional response mediated by the hypoxia-inducible factor (HIF) pathway and requires coactivation by the CBP and p300 transcriptional coactivators. Through a target-based high-throughput screen, we identified chetomin as a disrupter of HIF binding to p300. At a molecular level, chetomin disrupts the structure of the CH1 domain of p300 and precludes its interaction with HIF, thereby attenuating hypoxia-inducible transcription. Systemic administration of chetomin inhibited hypoxia-inducible transcription within tumors and inhibited tumor growth. These results demonstrate a therapeutic window for pharmacological attenuation of HIF activity and further establish the feasibility of disrupting a signal transduction pathway by targeting the function of a transcriptional coactivator with a small molecule.

PMID:
15261140
DOI:
10.1016/j.ccr.2004.06.009
[Indexed for MEDLINE]
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