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FEMS Microbiol Lett. 2004 Jul 15;236(2):183-9.

Characterization of florfenicol resistance among calf pathogenic Escherichia coli.

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1
Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, PR China.

Abstract

Three floR genes were cloned from calf pathogenic Escherichia coli strains, and the efflux-mediated accumulation of florfenicol in the floR gene-JM109 E. coli system was determined by HPLC. The floR genes resulted in a 1356-bp fragment covering the ORF in region 66-1280 coding for 404 amino acids. The common motifs of 12-transmembrane segments efflux pumps family were conserved in the deduced floR amino acid sequences. HPLC results indicated a significant difference in florfenicol accumulation between florfenicol-resistant strains and the susceptible strains, which was almost reversed by the addition of a proton motive force blocker. These results suggest that the florfenicol resistance mediated by the floR gene involves active efflux of florfenicol.

PMID:
15251195
DOI:
10.1016/j.femsle.2004.05.013
[Indexed for MEDLINE]
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