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J Chromatogr A. 2004 Jun 11;1039(1-2):105-11.

Detection of hypophosphite, phosphite, and orthophosphate in natural geothermal water by ion chromatography.

Author information

1
Department of Chemistry and Biochemistry, California State University, 5151 State University Drive, Los Angeles, CA 90032-8202, USA.

Abstract

Current doctrine states that phosphorus is incorporated into cells in the pentavalent(V) oxidation state as orthophosphate. However, recent studies show that microorganisms contain enzymes used to metabolize reduced forms of phosphorous, including phosphite(III) and hypophosphite(I), which suggests that there is a natural source for these chemical species. This paper will discuss suppressed conductivity ion chromatography methods developed to detect hypophosphite, phosphite, and orthophosphate in a geothermal water matrix containing fluoride, chloride, bromide, nitrate, hydrogen carbonate and sulfate. All peaks were clearly resolved, and calibrations were linear with estimated 3sigma detection limits of 0.83, 0.39, and 0.35 microM for hypophosphite, phosphite, and orthophosphate, respectively.

PMID:
15250410
DOI:
10.1016/j.chroma.2003.11.056
[Indexed for MEDLINE]

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