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Brain Res Bull. 2004 Jul 15;63(6):499-507.

Localization of G protein-coupled receptor kinases (GRKs) in the avian retina.

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Departamento de Neurobiologia, Universidade Federal Fluminense, Cx. Postal 100180, Niterói RJ 24001-970, Brazil.


G protein-coupled receptor kinases (GRKs) are enzymes involved in agonist-dependent regulation of G protein-coupled receptors. In the present work, we characterized, by immunohistochemistry, the presence of GRKs 2, 3 and 5 in the chick retina, a tissue whose structure and neurochemistry are well known. These enzymes are expressed in specific cell types and regions of the retina. Immunoreactivity for GRK2 was found over photoreceptor inner segments, cell bodies of horizontal, amacrine and ganglion cells. Labeling for this enzyme was also observed over the two plexiform layers. Immunoreactivity for GRK3 was found in cell bodies of amacrine and ganglion cells. In plexiform layers, specific GRK3 immunoreactivity was observed only at the inner plexiform layer, where three bands of high labeling were detected. In contrast to GRK2 and 3, intense immunoreactivity for GRK5 was observed only over Müller cells. Occasionally, labeled amacrine cell bodies were also observed. These results suggest that GRKs 2, 3 and 5 are expressed and involved in the physiology of specific cells types of the retina. They also suggest that receptor-GRK specificity may be determined by the co-expression of the receptor and the kinase within individual cell populations in this tissue.

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