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Diabetologia. 2004 Jul;47(7):1331-1333. doi: 10.1007/s00125-004-1452-1. Epub 2004 Jul 10.

Neonatal oral administration of DiaPep277, combined with hydrolysed casein diet, protects against Type 1 diabetes in BB-DP rats. An experimental study.

Author information

1
Department of Cell Biology, Immunology Section, Groningen University, A. Deusinglaan 1, 11th Floor, Room 1121, 9713 AV, Groningen, The Netherlands. s.brugman@med.rug.nl.
2
Department of Cell Biology, Immunology Section, Groningen University, A. Deusinglaan 1, 11th Floor, Room 1121, 9713 AV, Groningen, The Netherlands.
3
Department of Medical Microbiology, Molecular Virology Section, Groningen University, The Netherlands.
4
Peptor, Rehovot, Israel.

Abstract

AIMS/HYPOTHESIS:

Environmental factors such as diet and bacterial antigens play an important role in the onset of Type 1 diabetes. Different self-antigens are suggested to play a role in the development of diabetes. Antibodies against the 60-kDa heat shock protein 60, which have a high homology to bacterial heat shock protein 65, have been found in the circulation at the onset of diabetes in humans and in pre-diabetic NOD-mice. One of the immunodominant epitopes in autoimmune diabetes is p277, a specific peptide of human heat shock protein 60 corresponding to positions 437-460. In this study we investigated whether neonatal oral administration of DiaPep277 (a synthetic peptide analogue of p277) affected the development of diabetes in the BioBreeding-Diabetes Prone (BB-DP) rat, and whether this could potentiate the effect of a protective hydrolysed casein-diet.

METHODS:

BB-DP rats were orally inoculated once per day with placebo or DiaPep277 at days 4, 5, 6 and 7 of life. At the age of 21 days rats were weaned on to a conventional, cereal-based diet or on to the hydrolysed casein-diet.

RESULTS:

The development of diabetes in animals receiving DiaPep277 in combination with the hydrolysed casein-diet was delayed by 17 days, and a relative reduction of the incidence by 64% was seen. Non-diabetic animals did not show any sign of insulitis.

CONCLUSIONS/INTERPRETATION:

Short-term neonatal feeding with p277 in early life, combined with diet adaptation, appears to provide a procedure to significantly reduce the development of Type 1 diabetes in later life.

PMID:
15248047
DOI:
10.1007/s00125-004-1452-1
[Indexed for MEDLINE]
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