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J Clin Microbiol. 2004 Jul;42(7):3309-12.

A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements.

Author information

1
Genomic Research Laboratory, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland. patrice.francois@genomic.ch

Abstract

We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

PMID:
15243102
PMCID:
PMC446242
DOI:
10.1128/JCM.42.7.3309-3312.2004
[Indexed for MEDLINE]
Free PMC Article
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