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J Antimicrob Chemother. 2004 Aug;54(2):451-5. Epub 2004 Jul 1.

Epidemiological characteristics and molecular basis of fluoroquinolone-resistant Neisseria gonorrhoeae strains isolated in Korea and nearby countries.

Author information

1
Department of Laboratory Medicine, Research Institute of Bacterial Resistance and Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea.

Abstract

OBJECTIVES:

This study was performed to examine the cause of the increase in quinolone-resistant Neisseria gonorrhoeae (QRNG) observed in Korea.

METHODS:

The antimicrobial susceptibilities of 190 isolates of gonococci from Korea in 2000 were examined by NCCLS methods, and subsets of these isolates underwent mutation analysis of the quinolone resistance-determining regions (QRDRs) of gyrA and parC. Molecular epidemiological characterization of 25 Korean isolates and 54 isolates from overseas was performed by pulsed-field gel electrophoresis (PFGE) and the results compared.

RESULTS:

Most (172, 90.5%) of the 190 gonococci tested displayed reduced susceptibility to ciprofloxacin. All strains with high-level ciprofloxacin resistance (ciprofloxacin MIC >/= 4 mg/L) contained a double amino acid alteration at the 91 and 95 positions in the QRDR of GyrA and a single alteration in ParC. PFGE types of high-level QRNG in Korea were mostly different from those of other nearby countries.

CONCLUSIONS:

These results suggest that the observed increase in ciprofloxacin-resistant isolates is due to the mutation and spread of Korean multiclonal isolates rather than importation from overseas.

PMID:
15231766
DOI:
10.1093/jac/dkh345
[Indexed for MEDLINE]

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