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Genes Dev. 2004 Jul 1;18(13):1618-29.

Intracellular transcription of G-rich DNAs induces formation of G-loops, novel structures containing G4 DNA.

Author information

1
Department of Genetics, Yale University School of Medicine, New Haven, Conneticut 06520, USA.

Abstract

We show that intracellular transcription of G-rich regions produces novel DNA structures, visible by electron microscopy as large (150-500 bp) loops. These G-loops are formed cotranscriptionally, and they contain G4 DNA on one strand and a stable RNA/DNA hybrid on the other. G-loop formation requires a G-rich nontemplate strand and reflects the unusual stability of the rG/dC base pair. G-loops and G4 DNA form efficiently within plasmid genomes transcribed in vitro or in Escherichia coli. These results establish that G4 DNA can form in vivo, a finding with implications for stability and maintenance of all G-rich genomic regions.

PMID:
15231739
PMCID:
PMC443523
DOI:
10.1101/gad.1200804
[Indexed for MEDLINE]
Free PMC Article

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