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J Chromatogr A. 2004 Jun 25;1040(2):205-8.

Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography.

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Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China.


High-speed counter-current chromatography was successfully used for the first time for the preparative separation and purification of paeoniflorin from the Chinese medicinal plant Paeonia lactiflora Pall. using a two-phase solvent system composed of n-butanol-ethyl acetate-water (1:4:5, v/v) in a single run. From 160 mg of the crude sample containing 22.0% paeoniflorin, 33.2 mg of paeoniflorin was yielded at 98.2% purity as determined by HPLC analysis. The recovery of paeoniflorin was 94.3%.

[Indexed for MEDLINE]

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