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J Am Chem Soc. 2004 Jul 7;126(26):8078-9.

Adaptability at a protein-DNA interface: re-engineering the engrailed homeodomain to recognize an unnatural nucleotide.

Author information

1
Department of Chemistry, University of California, Berkeley, CA 94720, USA.

Abstract

We explore the adaptability at a protein-DNA interface using phage display to re-engineer a homeodomain to specifically recognize a chemically synthesized, unnatural nucleotide. The engineered homeodomain preferentially binds to the modified DNA with affinity and specificity similar to those of natural homeodomains.

PMID:
15225024
DOI:
10.1021/ja048113w
[Indexed for MEDLINE]

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